Coding

Part:BBa_K656011:Design

Designed by: Evan Clark, Julius Ho, Eli Moss, Jesse Palmer   Group: iGEM11_Brown-Stanford   (2011-09-19)

cscB Sucrose Symporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 310
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 310
    Illegal NgoMIV site found at 487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 531


Design Notes

Our intent with this part was to pair it with a promoter for Anabaena 7120 (part BBa_K656010) to enable sucrose secretion in that organism. For reference, see construct part BBa_K656012. Our part is designed to secrete sucrose because other sugars like fructose and glucose are readily metabolized by the organism itself, and therefore less likely to be available for secretion. Our bigger picture goal would be to use the sucrose secreted by Anabaena to provide energy for other organisms like modified E. coli W.

Source

e. coli W

References